The culture of resistance, with its characteristics of flexibility, self-confidence, awakening, Islamism, independence, anti-oppression, anti-arrogance, and unity of the Islamic world, has its roots in God-centeredness and is one of the achievements of the Islamic Revolution. This culture, in the form of Islamic, jihadi, and liberation movements, has been a serious threat to the survival and interests of the colonizers. Therefore, the leaders of arrogance have always sought to destroy it by adopting various strategies and tactics. The present study, with a descriptive-analytical approach and inspired by the monotheistic vision of Imam Khomeini (RA), examines the impact of the Islamic Revolution on the expansion and development of the culture of resistance in the Middle East. The findings and results of the research show that understanding the culture of resistance means understanding the essence of the Islamic Revolution and understanding the depth of the constructive thinking of its leaders, who have always sought the unity and integrity of the Islamic world and the revival of Islamic civilization, and consider it an achievement that, by expanding and developing it, can defend the essence and geography of the Islamic world and force the enemies of the Islamic nation to surrender.